ddbtp datp mix (Jena Bioscience)
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Jena Bioscience
ddbtp datp mix
Ddbtp Datp Mix, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ddntp+mix/ddNTP+Bundle/pm42716011-778-35-46
Average 94 stars, based on 10 article reviews
Ddbtp Datp Mix, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ddntp+mix/ddNTP+Bundle/pm42716011-778-35-46
Average 94 stars, based on 10 article reviews
ddbtp datp mix - by Bioz Stars,
2026-10
94/100 stars
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other:Article Title: Click-chemistry-aided quantitation and sequencing of oxidized guanines and apurinic sites uncovers their transcription-linked strand bias in human cells Article Snippet: For ddNTP blocking, gDNA was first incubated with 1 μL ENDOIV (NEB, 10 U/μL) in 1x ThermoPol Buffer (NEB) in a final volume of 20 μL for 20 min at 37°C. Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome Article Snippet: The plasmid pEGFP-W (ref. ), derived from pEGFP-N1 (Addgene, 6085-1), was purified from BL21(DE3) Escherichia coli (NEB, C2527H) using a Qiagen plasmid maxi prep kit (Qiagen, 12162) and digested with BamHI-HF (NEB, R3136S) and HindIII-HF (NEB, R3104S) restriction enzymes, generating sticky ends for subsequent ligation. Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome. Article Snippet: Specifically, 20 U of each enzyme was added to 5 μg of the plasmid along with 5 μl of 10× rCutsmart buffer (NEB, B6004S) in a final volume of 50 μl, followed by incubation for 1 h at 37 °C. Incubation:Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome. Article Snippet: For ddNTP blocking, gDNA was first incubated with 1 μl of ENDOIV (NEB, M0304L) in 1× ThermoPol buffer (NEB, B9004S) in a final volume of 20 μl for 20 min at 37 °C. .. Then, 2.5 μl of Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome. Article Snippet: Undesired background modifications were blocked by adding 1 μl of ENDOIV (NEB, M0304L) and 500 μM 8-HQ to 5 μg of gDNA, freshly extracted from HAP1 pellets, in 1× ThermoPol buffer (NEB, B9004S) in a final volume of 20 μl, and incubating the mixture for 20 min at 37 °C. .. Then, 2.5 μl of Article Title: Click-chemistry-aided quantitation and sequencing of oxidized guanines and apurinic sites uncovers their transcription-linked strand bias in human cells Article Snippet: Undesired background modifications were blocked by adding 1 μL ENDOIV (NEB, 10 U/μL) and 500 μM 8-HQ to 5 μg of gDNA, freshly extracted from HAP1 pellets, in 1x ThermoPol Buffer (NEB) in a final volume of 20 μL, and incubating the mixture for 20 min at 37°C. .. 2.5 μL Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome Article Snippet: Fluorescence was recorded with an Infinite Pro M200 plate reader (Tecan) using the following settings: λ Ex = 580 nm, λ Em = 610 nm, gain = optimal, 25 flashes, 20 μs of integration time and 25 °C. .. Then, 2.5 μl of Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome Article Snippet: For ddNTP blocking, gDNA was first incubated with 1 μl of ENDOIV (NEB, M0304L) in 1× ThermoPol buffer (NEB, B9004S) in a final volume of 20 μl for 20 min at 37 °C. .. Then, 2.5 μl of |